Antonio Conte's Chelsea were rocked by Marcus Rashford's early goal and a deflected Ander Herrera strike soon after half-time at Old Trafford delivered a major blow to their title ambitions.
They trail fourth place Manchester City by four points and have a game in hand.
"We all know the next game and how you go for the final or you don't go and for this reason, you must put 200 percent to reach the final", he said.
"This league is not closed".
"Obviously we lost away but I think our first half there [at Stamford Bridge] was maybe our best of the season and I think we've got nothing to be afraid of".
"He's not scoring enough goals, he's missing a bit and he had an opportunity and he didn't score and he looks like he was not very confident but the kid played very well and again, great experience to play well against such a team like Chelsea".
United manager Jose Mourinho started with top scorer Zlatan Ibrahimovic on the bench but delivered a tactical masterclass against his former club, largely nullifying the threat from the usually risky Eden Hazard.
"I think Marcus is a great player", Italy full-back Darmian said. "We can be very happy".
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"There are only six games left, and we are still in a good position".
"If we are able to win I think we must be proud for this, otherwise we must clap another team".
Apparently smelling blood and keen on ratcheting up the pressure on Chelsea, Spurs boss Pochettino has chose to unleash the mind games, and has started to fire a couple of shots.
"Next week we've got Middlesbrough at home, that's a massive game and three points would boost our season", Pugh told the club website.
Herrera was tasked with man-marking Eden Hazard throughout the match and Neville picked the Spaniard as the Man of the Match for Sky Sports after the game.
And yesterday's crunch game at Old Trafford has officially reignited the title race.
United's Ashley Young then blasted over from a promising position on the stroke of halftime but the hosts did not have to wait long after the break to double their advantage.
Herrera, unsurprisingly, won the man of the match award for his performance in Manchester United's 2-0 win against Chelsea after his contribution both going forward and defending his own goal.
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