In March of this year, the European Court of Human Rights said the decision of Britain's High Courts to allow doctors to stop treating Alfie did not violate his human rights.
Little Alfie lost his battle with a degenerative brain disease in the early hours of Saturday morning his parents Tom and Kate confirmed today.
Alfie's parents heavily criticized both the outcome of the court case and the medical staff at Alder Hey Children's Hospital, where Alfie had been since December 2016, calling their son a "prisoner".
On April 23, he tweeted, "Moved by the prayers and enormous solidarity shown little Alfie Evans, I renew my appeal that the suffering of his parents may be heard and that their desire to seek new forms of treatment may be granted".
The pope's tweets about the boy drew significant attention, prompting comparisons to Charlie Gard, a British baby who died past year despite his parents' fight - with the expressed support of Pope Francis and President Donald Trump - to keep him on life support. He was 23-months-old.
But the boy's parents fought in court, all the way up to the British Court of Appeal, to keep him on life support. "We are heart broken", his parents posted on Facebook.
All of us feel deeply for Alfie, Kate, Tom and his whole family and our thoughts are with them. "This has been a devastating journey for them", it said.
United Kingdom Judge, Justice Anthony Hayden, said the ruling represented "the final chapter in the life of this extraordinary boy".
Porn star Stormy Daniels sues US President Donald Trump for defamation
And in a March interview with 60 Minutes' Anderson Cooper, as CNN reported at the time, Daniels claimed that she was threatened . The Enquirer's parent company, American Media Inc., is run by David Pecker, a friend and ally of Trump's.
His parents, who live in Bootle, wanted to fly the toddler to an Italian hospital, but this was rejected by doctors who said continuing treatment was "not in Alfie's best interests".
Before his death a prayer vigil for Alfie Evans was held at the Vatican.
Alfie's life-support machine was switched off on Monday night. "Thankyou everyone for all your support", the post said.
The father of gravely ill Alfie Evans, the 23-month-old boy whose plight has drawn worldwide attention, said on Thursday he wanted to build bridges with staff at the British hospital he has been battling in the courts over his son's treatment.
On Friday, his parents said they would work with doctors to ensure their son had the dignity and comfort he needs.
Merseyside Police launch an investigation into "instances of verbal abuse and acts of intimidation" among protesters outside the hospital.
The RC Archbishop of Liverpool, Malcolm McMahon, said: "All who have been touched by the story of this little boy's heroic struggle for life will feel this loss deeply". But as a Christian Alfie has the promises of God, who is love, to welcome him into his heavenly home.