The son of a retired police officer, Jadhav's neighbours remember him as a shy but helpful man who was good at sport and studies, according to Indian media reports.
The wife and mother greeted media persons with a namaste after arriving at the ministry but did not respond to their questions.
A security official said the two women will meet with Jadhav in the presence of three Indian diplomats.
Jadhav has been languishing in a Pakistani jail ever since his arrest past year over allegations that he is a spy for the Research and Analysis Wing (RAW), India's premier external intelligence agency.
The mother and wife of Kulbhushan Jadhav held a meeting with him after Pakistani government accepted India's request.
Pakistan Rangers, Anti-Terrorism Squads (ATS) and sharp shooters were deployed at the roof tops, in view of the meeting. He said the case was still pending before the International Court of Justice, and that Jadhav had requested clemency from Pakistan's army chief, Gen. Qamar Javed Bajwa.
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Opponents say such a law would damage doctor-patient relationships and does nothing to improve lives of those with Down syndrome. The law also says doctors who perform such abortions could face fourth degree felony charges and lost their medical license.
India says Jadhav is a former naval officer but rejects the charges against him as baseless, saying he has no connection with the government.
Pakistan claims its security forces arrested Jadhav from its restive Balochistan province after he reportedly entered from Iran. The ICJ halted his execution on India's appeal pending the final verdict by it. If Bajwa declines, he could then seek a pardon from Pakistan's president. New Delhi has also accused Islamabad of violating the Vienna Convention by denying consular access. New Delhi blames Islamabad for fuelling a Muslim separatist insurgency in the divided Kashmir region, which remains the primary source of tensions between the nuclear-armed nations.
Jadhav's wife and mother met him for the first time after his detention in March 2016, but they were seated across a glass partition at the Pakistan Foreign Office. Congress said the meeting was deeply unsatisfactory.
"On the other hand, the entire episode unfolded was deeply unsatisfactory".
"The lives of Kashmiris have no value and we have no rights just because [we] are struggling against India in a fight for freedom".
Iit took place for around 40 minutes, that too they were separated by a glass barrier and what they spoke was also recorded.