Mount Agung was spewing smoke up to 1500 meters above its summit, two times higher than on Tuesday, when the smoke has forced the exodus of the people living near the mountain.
Authorities have kept Mt Aung's emergency status at level 3.
Authorities raised the alert to the highest level and ordered the evacuation of people living within a 12-kilometer radius around Mount Agung, forcing some 130,000 residents to leave their homes in September and October.
Global flights in and out of Bali have been grounded after Mount Agung erupted for the second time in a week sending travel plans into chaos.
However, Batik Air, Garuda, and Indonesia AirAsia are still operating.
Bali is an important tourist centre and the worldwide airport of Denpasar, the capital of the island, is operating normally for the moment, even if some airlines chose to cancel their flights.
People intending to travel should check the airline's website.
30 p.m. local time (1130 GMT), spreading ash 1,500 meters to the sky, higher than that at the first eruption on November 21 of 700 meters, spokesman of national disaster management agency Sutopo Purwo Nugroho said.
Volcano erupts again in Indonesia's Bali
Camera IconA volunteer puts a mask on a woman due to ash in the air from Mt. Agung volcano.
'Following an eruption of the Mount Agung volcano in Bali, Indonesia, it is not now safe to operate flights around Denpasar Airport, ' the airline said in a statement.
It was considered minor, as was an earlier eruption on Tuesday.
Tens of thousands of people have fled their homes in recent months over fears of potential destruction an eruption could bring.
An exclusion zone around the volcano extends 7.5km from the crater in places.
More than 1,000 people were killed when the mountain last erupted in 1963.
Indonesia is located on the "ring of fire" in the Pacific, where the collision of tectonic plates causes frequent earthquakes and a major volcanic activity.
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